tissue culture is vegatatively propagated by?

Plant tisue culture is a vegetative method ( micropropagation technique) which we use to produce resistanat variety plants, plants with more yield and sustainability. it can be vegetatively propagated from the shoot,root and any part of the plant which we name as the explant. The explant must be sterilised before taken for tissue culture and the procedure and the laboratory area should be completely sterile.shoot tip culture and nodal culture are made to form callus(undifferentiated mass of cells) which then grows into a copact planlet when suspended in suitable medium (liquid or solid). After attaining proper growth, the plantlet is alowed to grow in primary hardening and then transferred for secondary hardening which is distributed to the farmers and most importantly it should be devoid of infection.